Chemokine receptor 4 gene silencing blocks neuroblastoma metastasis in vitro.
NEURAL REGENERATION RESEARCH(2014)
摘要
This study investigated the effects of small interfering RNA (siRNA)-mediated silencing of chemokine receptor 4 (CXCR4) on the invasion capacity of human neuroblastoma cell line SH-SY5Y in vitro. Three siRNAs targeting CXCR4 were chemically synthesized and individually transfected into SH-SY5Y cells. Expression of CXCR4 mRNA and protein was significantly suppressed in transfected cells by all three sequence-specific siRNAs compared with control groups. Furthermore, the invasion capacity of SH-SY5Y cells was significantly decreased following transfection with CXCR4-specific siRNA compared with the control groups. These data demonstrate that down-regulation of CXCR4 can inhibit in vitro invasion of neuroblastoma.
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关键词
nerve regeneration,chemokine receptor 4,small interfering RNA,neuroblastoma,invasion,Transwell chamber,liposome,NSFC grant,neural regeneration
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