Precise manipulation of bacterial chromosomes by conjugative assembly genome engineering
NATURE PROTOCOLS(2014)
摘要
The Isaacs laboratory provides its protocol for CAGE; this method harnesses bacterial conjugation to precisely engineer E. coli genomes to create chimeric strains. A single round of CAGE can be completed in 1 week.
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关键词
Bacterial techniques and applications, Synthetic biology, Genomic engineering
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