Biooxidation of n-butane to 1-butanol by engineered P450 monooxygenase under increased pressure.

•We developed a fusion protein variant CYP153A for the conversion of n-butane to 1-butanol.•Maximum yield of 3.2g/L butanol after 20h reaction time.•Under 15bar pressure a total amount of 4.53g/L butanol was obtained.•Using the engineered monooxygenase system and the high pressure reactor a fivefold enhanced product concentration could be accomplished.•For a commercial feasible process butanol concentration has to be further improved at least ten times.