A novel surface-confined glucaminium-based ionic liquid stationary phase for hydrophilic interaction/anion-exchange mixed-mode chromatography.

•A novel surface-confined glucaminium-based IL stationary phase was prepared.•The column presented good chromatographic performances under HILIC mode.•The column possessed a column efficiency of 80,000plates/m.•It exhibited a hydrophilic interaction/anion-exchange mixed-mode retention mechanism.