Engineered oligosaccharyltransferases with greatly relaxed acceptor-site specificity
NATURE CHEMICAL BIOLOGY(2014)
摘要
Production of eukaryotic proteins in bacteria is limited by the specificity of the bacterial oligosaccharyltransferases that perform N -linked glycosylation. ‘GlycoSNAP’ simplifies the study of these enzymes, leading to mutants with relaxed specificity.
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nature chemical biology, science
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