A new structure determination method of lectins using a selenium-containing sugar ligand.
LECTINS: METHODS AND PROTOCOLS(2014)
Abstract
Phase determination is essential for solving X-ray crystal structures of proteins and their complexes. Conventional phase-determination methods using heavy atoms (Pt, Au, Hg, etc.) or the selenium (Se) atom are routinely utilized in structure determination of protein crystals. Here, we describe an alternative phase-determination method for proteins such as lectins in which a Se-containing glycan is used as a ligand. In this technique, the Se atoms are simply introduced into the protein crystal as a complex, and the phase of the protein can be determined using anomalous signals from the Se-containing sugar.
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Key words
X-ray crystallography,Phase determination,Anomalous dispersion,Glycan synthesis,Lectin
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