Zebrafish embryotoxicity test for developmental (neuro)toxicity: Demo case of an integrated screening approach system using anti-epileptic drugs

•The zebrafish embryotoxicity test (ZET) was applied using integrated multiple endpoints.•Morphology, motor activity, histopathology, kinetics and gene expression were studied.•A series of anti-epileptic drugs showed specific sensitivity for each endpoint.•This test strategy may contribute to elucidate the mode of action for developmental toxicity in ZET.•This test strategy may contribute to better define the applicability domain of the ZET.