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根癌农杆菌介导灰毡毛忍冬遗传转化体系的建立

刘国花,刘奕清,陈泽雄,刘周江

Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials（2013）

Cited 23|Views39
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Abstract
目的:在建立灰毡毛忍冬再生体系的基础上,建立其遗传转化体系.方法:组织培养方法培养幼苗,根癌农杆菌介导方法转化外植体,gus染色和PCR检测报告基因.结果:侵染时间8 min能获得较理想的转化效果.35 mg/L的卡那霉素和600 mg/L头孢霉素能得到很好的筛选效果,起主导作用的因素是卡那霉素浓度,其不同的浓度间抗性芽诱导率达到了极显著差异.对转基因植株进行gus染色和PCR检测,结果表明gus基因已整合到灰毡毛忍冬基因组中.结论:首次建立了根癌农杆菌(Agrobacterium tumefaciens,EHA105菌株)介导的叶片为受体的遗传转化体系.
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Key words
plant leaves,polymerase chain reaction
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