Glycomyces Artemisiae Sp Nov., An Endophytic Actinomycete Isolated From The Roots Of Artemisia Argyi

An endophytic actinomycete strain, IXS4(T), was isolated from the root of Artemisia argyi, a medicinal plant collected from Yesanpo located in Laishui county, Hebei province, China. The 16S rRNA gene sequence of strain IXST showed most similarity to Glycomyces mayteni YIM 61331(T) (98.23% 16S rRNA gene sequence similarity), Glycomyces sco-pariae YIM 56256(T) (98.00 %), Glycomyces sambucus E71(T) (97.90 %) and Glycomyces algeriensis NRRL B-16327(T) (97.10%). DNA DNA hybridization values between strain IXS4(T) and the closely related type strains were well below 70%. The strain also showed a number of physiological and biochemical characteristics that were distinct from the closely related species. The strain contained MK-10(H-2) and MK-11(H-0) as the detected menaquinones. The peptidoglycan was mainly meso-dianninopimelic acid and the whole-cell sugars contained galactose, glucose, mannose, xylose and ribose. The major cellular fatty acids were iso-C-14:0, iso-C-16:0, anteiso-C-15:0 and anteiso-C-17:0. Based on the genetic and phenotypic properties, it is proposed that strain IXS4(T) represents a novel species of the genus Glycomyces, with the name http://dx.doi.org/10.1601/nm.7671 Glycomyces artemisiae sp. nov. The type strain is IXS4(T) (=HBUM178000(T)=CGMCC 4.7067(T)=NBRC 109773(T)).