Bacterioplanes sanyensis gen. nov., sp. nov., a PHB-accumulating bacterium isolated from a pool of Spirulina platensis cultivation

A Gram-negative, poly-3-hydroxybutyrate-accumulating rod bacterium, strain GYP-2 T , was isolated from a pool of marine Spirulina platensis cultivation, Sanya, China. Growth was observed at 10–45 °C and pH 6–10 in the presence of 1–10 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the new isolate belonged to Gammaproteobacteria and displayed 93.8–95.3 % 16S rRNA gene sequences similarities to members of the genera Thalassolituus , Oleibacter , and Oceanobacter , but house-keeping gene gyr B (encode DNA gyrase beta subunit) demonstrated that the new isolate was distantly related to Thalassolituus , Oleibacter , and Oceanobacter species (only 77–83 % gene gyr B sequences similarities).The G+C content of genomic DNA was 55 mol%. The major respiratory quinone was Q-9, while that for Oceanobacter kriegii LMG 6238 T was Q-8. Major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, and phosphatidylethanolamine. On the basis of its physiological, chemotaxonomic, and molecular properties, strain GYP-2 T is suggested to represent a novel species of a new genus in Gammaproteobacteria , for which the name Bacterioplanes sanyensis gen. nov., sp. nov. is proposed. The type strain is GYP-2 T (=CGMCC 1.12392 T =KCTC 32220 T ).