Frequency-Tunable Two-Color Ultrafast Fiber Laser for Nonlinear excitation of NADH and FAD

We will present our latest innovations about ultrafast fiber lasers and show how multiphoton microscopy can benefit from these developments. Wavelengths around 900 nm and pulse durations as short as 100 fs remain a challenge for fiber lasers. Here we present a two-color femtosecond fiber laser system with synchronous outputs. One arm emits pulses at a central wavelength of 780 nm and the novel second laser arm is continuously tunable in its central wavelength between 810 nm and 950 nm. This allows the independent excitation of NADH and FAD and therefore enables optical metabolism and oxygen imaging of cells via FLIM and PLIM measurements.