Highly active fractions of the medicinal leech recombinant destabilase-lysozyme]
Biomedit͡sinskai͡a khimii͡a(2014)
Abstract
From the highly purified but lowly active recombinant protein Destabilas-Lysozyme (Dest-Lys) by use cation-exchange column TSK CM 3-SW chromatography, it was separated non-active fraction IV, contained 90% of protein. Fractions I, II and III, represented proteins with lysozyme and isopeptidase activities. Their lysozyme activity correlates with the activity of natural Des-Lys. The ratio of the activities in fractions I - III is such, that maximal lysozyme activity is concentrated in fraction III, isopeptidase - in fraction I. It is discussed the possibility of Dest-Lys different functions regulation is depended on the formation of protein complex forms.Abstract available from the publisher.
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Key words
destabilase-lysozyme of hirudo medicinalis,forms of recombinant enzyme,lysozyme and isopeptidase functions
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