New affinity-based probes for capturing flavonoid-binding proteins

Flavonoid-bearing probes have been designed and synthesized to explore their ability to selectively capture target proteins or biosynthetic enzymes under oxidative activation. A proof-of-concept study using biotinylated (epi)catechin-bearing affinity-based probes herein demonstrates the ability of these probes to capture the LDOX flavonoid enzyme using sodium periodate as the oxidant.