Production of monoclonal antibodies for detection of Citrus leprosis virus C in enzyme-linked immuno-assays and immunocapture reverse transcription-polymerase chain reaction.

Journal of Virological Methods(2014)

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摘要
•MAbs were produced to the purified expressed coat protein of Citrus leprosis virus C.•MAbs C11, E10, F9 and G10 were selected to evaluate for the detection of CiLV-C.•MAbs G10 and C11 were recommended for detection of CiLV-C in DAS-ELISA.•MAb-G10 was recommended for detection of CiLV-C in DBIA and IC-RT-PCR.•IC-RT-PCR was more sensitive for detection of CiLV-C to DAS-ELISA and DBIA.
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关键词
Citrus leprosis virus C,Monoclonal antibody,DAS-ELISA,DBIA,IC-RT-PCR
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