Real-time fluorescence assays to monitor duplex unwinding and ATPase activities of helicases

Ali R Özeş,Kateryna Feoktistova,Brian C Avanzino,Enoch P Baldwin,Christopher S Fraser

NATURE PROTOCOLS（2014）

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摘要

Many physiological functions of helicases depend on their ability to unwind nucleic acid duplexes in an ATP-dependent fashion. Here, Özeş et al . describe a fluorescence assay to monitor in real time RNA duplex unwinding by DEAD-box helicases.

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关键词

Fluorescence resonance energy transfer, RNA, RNA-binding proteins, RNA probes

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