[ABO blood group genotyping based on amplification refractory mutation system].

OBJECTIVE:To develop a rapid, cost-effective and reliable method for ABO genotyping. METHODS:Six pairs of primers were designed for the amplification refractory mutation system (ARMS). Thirty peripheral blood DNA samples, derived from Chinese Han unrelated individuals, were genotyped by ARMS. The results were compared with the corresponding data obtained from serological methods and DNA sequencing. RESULTS:The ABO genotyping results using ARMS were perfectly in line with the serological methods and DNA sequencing. CONCLUSION:The method developed and optimized in this study is able to accurately and rapidly detect 28 kinds of ABO genotypes, indicative of a new approach applicable to clinical practice.