Part 1: Laboratory culture of Centroptilum triangulifer (Ephemeroptera: Baetidae) using a defined diet of three diatoms

•A reproducible culture method for a parthenogenetic mayfly species for use in toxicity tests.•The culture method has been used for over 9 generations.•Young have been successfully tested in toxicity tests with high control survival and growth.•Methods for culturing diatoms that can be used for culture and testing of Centriptilum triangulifer.