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Capture of activity-induced ultrastructural changes at synapses by high-pressure freezing of brain tissue

Daniel Studer,Shanting Zhao,Xuejun Chai,Peter Jonas,Werner Graber,Sigrun Nestel,Michael Frotscher

NATURE PROTOCOLS（2014）

Cited 25|Views10
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Abstract
This Protocol provides directions on using high-pressure freezing to prepare tissue samples for electron microscopy, avoiding the need for aldehyde fixation and ethanol dehydration.
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Key words
Transmission electron microscopy, Cellular neuroscience, Synaptic plasticity, Cellular imaging
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