Targeted deep sequencing of HIV-1 using the IonTorrentPGM platform.

Gustavo H Kijak,Eric Sanders-Buell,Elizabeth A Harbolick,Phuc Pham,Agnes L Chenine,Leigh Anne Eller,Kathleen Rono,Merlin L Robb,Nelson L Michael,Jerome H Kim,Sodsai Tovanabutra

Journal of Virological Methods（2014）

引用 5|浏览22

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摘要

•Exploration of suitability of IonTorrent PGM for targeted deep sequencing of HIV-1.•The error rates from misincorporation and in vitro recombination were <0.5%.•TDS provided an augmented view of HIV-1 evolution during acute infection.

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关键词

Targeted deep sequencing,Next-generation sequencing,HIV-1,Molecular evolution,IonTorrent

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