Aquamicrobium terrae sp. nov., isolated from the polluted soil near a chemical factory

A Gram-negative, aerobic, non-motile bacterial strain hun6 T isolated from the polluted soil near a chemical factory in northern Nanjing, China was investigated to clarify its taxonomic position. Growth of strain hun6 T occurred between 10 and 45 °C (optimum, 30 °C) and between pH 6.0 and 8.0 (optimum, pH 7.0). No growth occurred at NaCl concentrations greater than 5 % (w/v). The 16S rRNA gene sequence analysis indicated that strain hun6 T belongs to the genus Aquamicrobium . The sequence similarities of strain hun6 T to other type strains of Aquamicrobium genus were all below 98.5 %. The presence of ubiquinone-10, the predominant fatty acid summed feature 8 (C 18:1 ω7c and/or C 18:1 ω6c ) and C 19:0 cyclo ω8c , a polar lipid pattern with phosphatidylglycerol, phosphatidylcholine, diphosphatidylglycerol, phosphatidylethanolamine and phophatidylmonomethylethanoamine were in accord with the characteristics of the genus Aquamicrobium . The G+C content of the genomic DNA was determined to be 63.5 mol%. The results of DNA–DNA hybridization, physiological and biochemical tests and chemotaxonomic properties allowed genotypic and phenotypic differentiation of strain hun6 T from all known Aquamicrobium species. Therefore, strain hun6 T can be assigned to a new species of this genus for which the name Aquamicrobium terrae sp. nov. is proposed. The type strain is hun6 T (= CICC 10733 T = DSM 27865 T ).