The effect of hypochlorite on electrical surface properties of human blood lipoproteins]

Surface potential of human blood lipoproteins was studied by the use of Mn(2+)-ions as a positively charged spin probe. The calculated values of surface potential of low density lipoproteins (LDL) appeared to be -17.7 +/- 1.2 mV. It was shown that hypochlorite (OCl-) induced an increase of negative surface potential of LDL. Agarose gel electrophoresis was used for the comparative analysis of electrophoretic mobility of human blood lipoproteins of different classes. It was shown that the interaction of hypochlorite with lipoproteins caused the partial degradation of lipoproteins and the increase in surface charge. High density lipoproteins appeared to be more resistant to the action of OCl- than LDL and especially very low density lipoproteins.