Assignments of RNase A by ADAPT-NMR and enhancer

We report here backbone 1 H and 15 N assignments for ribonuclease A obtained by using ADAPT-NMR, a fully-automated approach for combined data collection, spectral analysis and resonance assignment. ADAPT-NMR was able to assign 98 % of the resonances with 93 % agreement with traditional data collection and assignment. Further refinement of the automated results with ADAPT-NMR enhancer led to complete (100 %) assignments with 96 % agreement with assignments by the traditional approach.