Enhanced heat stability and kinetic parameters of maize endosperm ADPglucose pyrophosphorylase by alteration of phylogenetically identified amino acids.
Archives of Biochemistry and Biophysics（2014）
Abstract
•Phylogenetic approach used for selection of key amino acids.•Mutations resulted in heat stability and improved allosteric properties of AGPase.•Combining several amino acid changes had an additive effect on heat stability.•Resulting enzyme (SH2-E) was heat stabile and no longer dependant on the activator.
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Key words
ADP-glucose pyrophosphorylase,Phylogenetic analysis,Heat stability,Allosteric effects,Phosphate inhibition,Zea mays
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