Tricomponent fusion complex comprising a viral antigen, a pentameric α-helical coiled-coil, and an immunoglobulin-binding domain as an effective antiviral vaccine.

The pentameric coiled-coil domain of cartilage oligomeric matrix protein (COMP) genetically fused to the Z domain of Staphylococcus aureus protein A, an immunoglobulin-binding domain (IBD), was evaluated as a viral antigen carrier complex. In a proof-of-concept study, recombinant Japanese encephalitis virus (JEV) E protein domain III (D3) was loaded onto the COMP-Z fusion protein by chemical conjugation, and the tricomponent complex generated, COMP-Z/D3, was evaluated for its vaccine efficacy in a mouse JEV infection model. Immunization with the complex conferred substantially greater protection against lethal JEV infection than the unloaded antigen. Next, a tricomponent complex was engineered in which the three molecular entities (the D3 antigen, COMP coiled-coil domain, and Z domain) were genetically connected in tandem to create the D3-COMP-Z tricomponent complex, or its reversal oriented construct, Z-COMP-D3. The fusion complexes were produced as inclusion bodies in Escherichia coli, but could be refolded to biologically active pentamers that retained the E protein antigenicity and the IBD function. Immunization with the refolded complexes conferred a high level of protection against lethal JEV infection, similar in efficacy to that of the tricomponent complex generated by chemical conjugation. These results demonstrate that the tricomponent complex, whether generated by chemical or genetic fusion, is a promising molecular design for the creation of effective subunit vaccines against viral infections.