Biological properties of live and inactivated influenza vaccines under experimental conditions]

Biological properties of influenza vaccines were studied on experimental animals inoculated intraperitoneally with regard to the vaccine effect on peritoneal exudate cells. The development of neutrophilia and inhibition of acid phosphatase activity in macrophages within the first 24 hours after intraperitoneal inoculation of influenza viruses or influenza vaccines were used for determination of the toxic effect of the inoculum. The values of subsequent later activation of acid phosphatase in macrophages allowed the immunogenic properties of the vaccines under study to be evaluated. The sensitizing properties of the virus antigens were assessed by the reaction of the lymphoid-macrophage system after multiple inoculations of the preparations. A correlation of changes observed in the experimental animals with reactogenicity and values of immunity against influenza in man and animals was established.