Determination of three triterpene alcohols in rat plasma after oral administration of pollen of Brassica campestris based on the utilization of fetal bovine serum as surrogate matrix.

•Fetal bovine serum was selected as the surrogate matrix and validated.•The derivatization of internal standard was developed and applied.•The method was developed for the first time.•The pharmacokinetics of DEH, MET and NOR was investigated.•This study provides the basis for the consumption of pollen of Brassica campestris.