Hydrophilic interaction liquid chromatography/positive ion electrospray ionization mass spectrometry method for the quantification of alprazolam and α-hydroxy-alprazolam in human plasma.

•HILIC chromatography enables rapid quantitation of alprazolam and α-hydroxy alprazolam in human plasma.•HILIC-ESI/MS enables the sensitive and selective quantitation of alprazolam and α-hydroxy alprazolam.•HILIC-ESI/MS was used to analyze samples obtained from patients treated for anxiety disorders.