The need for transparency and good practices in the qPCR literature

Stephen A Bustin,Vladimir Benes,Jeremy Garson,Jan Hellemans,Jim Huggett,Mikael Kubista,Reinhold Mueller,Tania Nolan,Michael W Pfaffl,Gregory Shipley,Carl T Wittwer,Peter Schjerling,Philip J Day,Mónica Abreu,Begoña Aguado,Jean-François Beaulieu,Anneleen Beckers,Sara Bogaert,John A Browne, Fernando Carrasco-Ramiro,Liesbeth Ceelen, Kate Ciborowski,Pieter Cornillie,Stephanie Coulon,Ann Cuypers,Sara De Brouwer, Leentje De Ceuninck,Jurgen De Craene, Hélène De Naeyer,Ward De Spiegelaere, Kato Deckers, Annelies Dheedene,Kaat Durinck, Margarida Ferreira-Teixeira,Annelies Fieuw,Jack M Gallup, Sandra Gonzalo-Flores,Karen Goossens,Femke Heindryckx,Elizabeth Herring,Hans Hoenicka,Laura Icardi,Rolf Jaggi,Farzad Javad,Michael Karampelias,Frederick Kibenge,Molly Kibenge,Candy Kumps,Irina Lambertz,Tim Lammens,Amelia Markey,Peter Messiaen,Evelien Mets,Sofia Morais,Alberto Mudarra-Rubio, Justine Nakiwala,Hilde Nelis,Pal A Olsvik,Claudina Pérez-Novo,Michelle Plusquin,Tony Remans,Ali Rihani,Paulo Rodrigues-Santos,Pieter Rondou,Rebecca Sanders, Katharina Schmidt-Bleek,Kerstin Skovgaard,Karen Smeets,Laura Tabera,Stefan Toegel, Tim Van Acker,Wim Van den Broeck,Joni Van der Meulen,Mireille Van Gele,Gert Van Peer,Mario Van Poucke,Nadine Van Roy,Sarah Vergult, Joris Wauman, Marina Tshuikina-Wiklander,Erik Willems,Sara Zaccara, Fjoralba Zeka,Jo Vandesompele

NATURE METHODS（2013）

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摘要

Two surveys of over 1,700 publications whose authors use quantitative real-time PCR (qPCR) reveal a lack of transparent and comprehensive reporting of essential technical information. Reporting standards are significantly improved in publications that cite the Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines, although such publications are still vastly outnumbered by those that do not.

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关键词

nature,quantitative pcr,qpcr,rna,cancer cells,polymerase chain reaction,pcr,publication,rt pcr,normalization,real time pcr

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