A multiparameter live cell imaging approach to monitor cyclic AMP and protein kinase A dynamics in parallel.
FLUORESCENT PROTEIN-BASED BIOSENSORS: METHODS AND PROTOCOLS(2014)
Abstract
Parallel detection of signaling activities allows us to correlate activity dynamics between signaling molecules. In this review, we detail a multiparameter live cell imaging method to monitor 3',5'-cyclic adenosine monophosphate (cAMP) levels and protein kinase A (PKA) activities in parallel.
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Key words
Parallel imaging,FRET,cAMP,Protein kinase A,Signal transduction
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