Opposite Regulatory Effects Of Ifn-Beta And Il-3 On C-Type Lectin Receptors, Antigen Uptake, And Phagocytosis In Human Macrophages

Novel role for type I IFN in regulating innate immunity through controlling macrophage phagocytosis and antigen uptake. CLRs are predominantly expressed in macrophages and myeloid DCs, where they play a key role in antigen recognition, scavenging, and host defense against pathogens. To identify novel immunoregulatory cytokines and networks involved in the control of these functions, we analyzed the coordinate effects of IFN- and IL-3 on CLR expression, antigen uptake, and phagocytosis in human MDMs and MDDCs. We report that these cytokines exert opposite regulatory effects on the expression of CLRs and endocytic/phagocytic activities of MDMs. Specifically, IFN- markedly inhibits the expression of MR and Dectin-1 during MDM differentiation and impairs the capacity of MDM to internalize antigens and phagocytose unopsonized Candida albicans. Conversely, IL-3 up-modulates MR, Dectin-1, and DC-SIGN, thus allowing more efficient uptake/phagocytosis. Interestingly, IL-3 counteracts the IFN- effect on antigen uptake/processing by fully restoring MR expression in IFN--primed MDMs. In contrast, the phagocytic activity is only partially restored as a result of the failure of IL-3 in counteracting IFN--induced Dectin-1 suppression. Notably, IFN--mediated impairment of CLR expression/function occurs in macrophages but not in MDDCs. These results identify IFN- and IL-3 as unrecognized regulators of CLR expression and function, unraveling a novel interaction between these cytokines instrumental for the regulation of the macrophage response to pathogens.