Gene Frequency Distribution of the BoLA-DRB3 Locus in Saavedreño Creole Dairy Cattle
Biochemical genetics(2004)
摘要
The objective of this study is to describe the gene frequency distribution of the bovine lymphocyte antigen (BoLA)-DRB3 locus in Saavedreño Creole dairy cattle and to compare it with previously reported patterns in other cattle breeds. One hundred and twenty-five Saavedreño Creole dairy cattle were genotyped for the BoLA-DRB3.2 allele by polymerase chain reaction and restriction fragment length polymorphism. Twenty-two out of 53 previously identified BoLA-DRB3.2 alleles were detected, with gene frequencies ranging from 0.4 to 16.8%. Seventy percent of the variation corresponded to the seven most frequent alleles ( BoLA-DRB3.2 *7, *8, *11, *16, *27, *36, and *37). The studied population exhibits a high degree of expected heterozygosity (h e = 0.919). The F IS index did not show significant deviation from Hardy-Weinberg equilibrium. However, the neutrality test showed an even gene frequency distribution. This result could be better explained assuming balancing selection instead of neutral or positive selection for one or a few alleles. In conclusion, the results of this study demonstrated that BoLA-DRB3.2 is a highly polymorphic locus in Saavedreño Creole dairy cattle, with significant variation in allele frequency among cattle breeds.
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关键词
BoLA-DRB3.2,Creole cattle,PCR-RFLP,genetic diversity
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