枸杞子、淫羊藿对衰老-年轻2BS融合细胞DNA合成的影响
Zhongguo Zhong xi yi jie he za zhi Zhongguo Zhongxiyi jiehe zazhi = Chinese journal of integrated traditional and Western medicine / Zhongguo Zhong xi yi jie he xue hui, Zhongguo Zhong yi yan jiu yuan zhu ban（2003）
Abstract
目的:研究枸杞子、淫羊藿水提液对衰老-年轻2BS融合细胞DNA合成的影响.方法:以人胚肺二倍体成纤维细胞国内标准株2BS为衰老模型,用细胞脱核和细胞融合技术,观察枸杞子、淫羊藿对衰老-年轻2BS融合细胞的DNA合成的影响.结果:2BS细胞分别在含0.025(V/V)枸杞子、淫羊藿水提液的DMEM培养液中,可连续培养至61.0±2.9、56.0±2.6代,对照组为49.0±2.6代(P＜0.01);衰老的2BS细胞分别用枸杞子、淫羊藿水提液处理2h后,进行细胞脱核并与年轻2BS细胞融合,其[3H]TdR掺入百分率明显高于对照组(P＜0.01).结论:枸杞子、淫羊藿均能促进衰老-年轻2BS融合细胞的DNA的合成速率,延长2BS细胞寿命.
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Cell Differentiation
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