ATP-generating creatine kinase: a new compound isolated from Viperidae venom]

D A Kuznetsov, M A Orlova, A G Berdieva,P Z Khasigov

Biomedit͡sinskai͡a khimii͡a(2003)

引用 23|浏览1
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摘要
This is a first report on the presence of creatine kinase, ATP-generating enzyme, in the venom of Vipera xanthia representing the Viperidae family of poisoning snakes. Kinetic and catalytic properties of 40 kD active monomer has been described with a following discussion on the issue of potential of the data listed for further research in either toxic coagulopathy molecular mode or enzyme application dealing with "thrombin-thrombin receptor" interaction studies. An enzyme purification procedure includes hydrophobic (phenyl-Sepharose), anion exchange (MonoQ) and affinity (ADP-Sepharose) chromatographic steps.
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