A novel and enantioselective epoxide hydrolase from Aspergillus brasiliensis CCT 1435: purification and characterization.

Lilian L Beloti,Bruna Z Costa,Marcelo A S Toledo,Clelton A Santos,Aline Crucello,Marianna T P Fávaro,André S Santiago,Juliano S Mendes,Anita J Marsaioli,Anete P Souza

Protein Expression and Purification（2013）

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Abstract

•Epoxide hydrolase from A. brasiliensis was detected and cloned, and recombinant EH was purified.•The conserved catalytic triad present in EHs is also present in AbEH.•AbEH hydrolyzed 66% of 8mM racemic styrene oxide (SO) and the remaining (S)-SO ee exceeded 99%.•In a water:isooctane biphasic system AbEH demonstrated a slightly enantioconvergent behavior.

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Key words

enantioselective epoxide hydrolase,aspergillus brasiliensis cct

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