[Construction of lentivirus vectors containing PNPLA3 gene and overexpression of PNPLA3 in Huh-7 cells].
Zhonghua gan zang bing za zhi = Zhonghua ganzangbing zazhi = Chinese journal of hepatology（2013）
Abstract
非酒精性脂肪性肝病(NAFLD)正在成为我国一个新的公共健康问题[1].PNPLA3 (patatin-like phospholipase domain-containing protein 3)基因I148M多态性与NAFLD密切相关,且此多态性参与了NAFLD的疾病进展[2-3].但是,其在NAFLD发病中的具体生物机制目前尚不明确.本实验利用基因重组技术,成功构建了携带PNPLA3基因野生型和I148M突变型的慢病毒载体,并感染人肝癌细胞株Huh-7细胞,建立了稳定过表达目的基因的细胞系,为深入研究PNPLA3基因的生物学功能提供了理想的细胞模型. 关键词：脂肪肝,非酒精性;慢病毒;Huh-7细胞;PNPLA3;I148M
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Key words
Fatty liver,nonalcoholic,Lentvirus,Huh-7 cells,PNPLA3,I148M
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