A fast and robust method for full genome sequencing of Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) Type 1 and Type 2.

Lise K Kvisgaard,Charlotte K Hjulsager,Ulrik Fahnøe,Solvej Ø Breum,Tahar Ait-Ali,Lars E Larsen

Journal of Virological Methods（2013）

引用 28|浏览9

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摘要

•A method for full genome sequencing of PRRSV Type 1 and Type 2.•Adequate for sequencing from primary material and propagated material.•A method for obtaining long range PCR amplicons is described.•Three next generation platforms are used for full genome sequencing.

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关键词

PRRSV,Full genome sequencing,Next generation sequencing,Illumina HiSeq2000,Roche 454 FLX,Ion Torrent PGM sequencer

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