Phosphonomycin: isolation from fermentation sources.
JOURNAL OF ANTIBIOTICS(1970)
摘要
Phosphonomycin, (-)-cis-1, 2-epoxypropylphosphonic acid, was isolated from fermentation sources as a crystalline calcium salt. Methods for ion-exchange adsorption, ion-exchange chromatography using both anion- and cation-exchange resins, gel-permeation chromatography, adsorption chromatography and solvent partition were investigated. The overall purification was about 5, 000 fold with a yield of 12 %, Conditins for ion-exchange chromatography were developed based on batch equilibration studies. Thin-layer chromatography was used to develop a chromatographic procedure with alumina. The molecular weight estimated from gel-permeation studies was somewhat higher than the true value. A number of other salts which remained amorphous were also prepared.
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关键词
fermentation
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