Validation of a cell-based ELISA as a screening tool identifying anti-alphavirus small-molecule inhibitors

Kevin B. Spurgers,Clarence R. Hurt,Jeffrey W. Cohen,Lori T. Eccelston,Cathleen M. Lind,Vishwanath R. Lingappa,Pamela J. Glass

Journal of Virological Methods（2013）

引用 16|浏览12

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摘要

•Presents a method for screening compounds for efficacy antiviral efficacy.•Demonstrate robustness of this assay.•Validate the use of this method by correlating the result with traditional assays.

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关键词

BSA,CHIKV,CPE,EEEV,ELISA,HRP,IC50,MAb,MOI,PBS,pfu,RT,S/B,SD,TCID50,TrD,USAMRIID,VEEV,WEEV

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