Effects of post-thaw incubation on motility, acrosomal integrity and in vitro fertilizing capacity of boar spermatozoa cryopreserved in 0.5 and 5 ml straws

The effect of post-thaw incubation (0 vs. 5h at 15°C) and straw size (5 vs. 0.5ml) on motility, acrosomal integrity and in vitro fertilizing (IVF) capacity of cryopreserved boar spermatozoa was studied. In samples assessed immediately after thawing, no differences were found between the two straw sizes. After 5h post-thaw incubation, all parameters, except polyspermy, decreased and, spermatozoa packaged in 5ml straws showed better functional and IVF parameters than these in 0.5ml straws.