Magnetofection of Human Somatic Cells with Magnetite and Cobalt Ferrospinel Nanoparticles
BULLETIN OF EXPERIMENTAL BIOLOGY AND MEDICINE(2013)
摘要
Superparamagnetic nanoparticles varying by their chemical composition and synthesis method were used to transfer DNA into somatic cells under the infl uence of constant magnetic field (method of magnetofection). Magnetite particles obtained by mechanochemical synthesis ensured higher expression of the marker gene GFP (evaluated by fluorescence intensity of the cell lysate) then particles of ferric oxide obtained by chemical co-precipitation and cobalt ferrospinel particles obtained by the mechanochemical method.
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关键词
superparamagnetic nanoparticles,magnetofection,human somatic cells
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