Competitive adsorption-desorption of IgM monomers-dimers on silica and modified silica surfaces.
Journal of Colloid and Interface Science(2013)
Abstract
Understanding competitive adsorption-desorption of proteins onto surfaces is an important area of research in food processing and biomedical engineering. Here, we demonstrate, how electrospray-differential mobility analysis that has been traditionally used for characterizing bionanoparticles, can be used for quantifying complex competitive adsorption-desorption of oligomeric proteins or multiprotein systems using monomers and dimers of IgM as a model example onto silica and modified silica surfaces. Using ES-DMA, we show that IgM dimers show a preference to stay adsorbed to different surfaces although monomers adsorb more easily and desorption rates of monomers and dimers of IgM are surface-type-dependent and are not significantly affected by shear. We anticipate that this demonstration will make ES-DMA a popular "label-free" method for studying multicomponent multi-oligomeric protein adsorption to different surfaces in the future.
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Key words
Electrospray,Differential mobility analysis,Protein,Competitive adsorption,Desorption,Bovine,Immunoglobulin,IgM,Silica surface,Gelatin
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