Enhanced loading of Fura-2/AM calcium indicator dye in adult rodent brain slices via a microfluidic oxygenator.

•A microfluidic oxygenator is used to deliver constant oxygen to rodent brain slices.•Device improves loading efficiency of calcium indicator Fura-2/AM into cells of slice.•Loading in slices from 1-year old mice was achieved.•Technique significantly expands the age range for which calcium studies are possible.