Cell penetrable human ScFv specific to influenza A virus matrix protein, M1, mitigates influenza severity

Event Abstract Back to Event Cell penetrable human ScFv specific to influenza A virus matrix protein, M1, mitigates influenza severity Fonthip Dong-din-on1, Potjanee Srimanote2, Tippawan Pissawong3, Angkasiya Monkong4, Preeda Lertwatcharasarakul4, Thaweesak Songserm4 and Wanpen Chaicumpa5* 1 Kasetsart University, Center for Agricultural Biotechnology, Thailand 2 Thammasat University, Graduate Program in Biomedical Science, Faculty of Allied Health Sciences, Thailand 3 Mahidol University, Department of Immunology, Faculty of Medicine Siriraj Hospital, Thailand 4 Kasetsart University, Veterinary Midicine, Thailand 5 Mahidol University, Department of Parasitology, Faculty of Medicine Siriraj Hospital, Thailand Matrix protein-1 (M1) is highly conserved across type A influenza viruses. This protein has many important functions in the viral replication cycle; thus it is one of the targets of novel anti-influenza. To study therapeutic efficacy of M1 specific, cell penetrable human single chain antibody (HuScFv) in mitigating severity of influenza in infected mice. E. coli derived-human single chain antibody fragments (HuScFv) specific to recombinant M1 of influenza A virus H5N1 (clade 1) was produced using a human antibody phage display library. HuScFv from selected huscfv-phagemid transformed E. coli clones were linked molecularly to a cell penetrating peptide, penetratin (PEN) and PEN-HuScFv were produced and purified. BALB/c mice were infected intranasally with mouse adapted-avian H5N1 virus (clade 2.3). They were then treated with M1 specific-PEN-HuScFv. Control infected mice received PBS treatment. Internal organs (lung, brain, spleen, liver and kidney) were collected. Tissue viral loads were determined by real time RT-PCR. Histopathology of the tissues was also examined. Infected BALB/c mice that received M1 specific-PEN-HuScFv had reduced viral loads and histopathological features in tissues compared to the controls. M1 specific-PEN-HuScFv could mitigate severity of influenza in mice infected with A H5N1 of the heterologous clade. Cross therapeutic efficacy of the transbodies on influenza caused by different virus subtypes remains to be evaluated. Acknowledgements This research work was financially supported by grant no. DPG5380001 of the Thailand Research Fund (TRF) and the National Research University (NRU) Project, Office of Higher Education Commission (CHE), Ministry of Education, Thailand. Fonthip is a TRF scholar of the Royal Golden Jubilee Ph.D. Program. References 1. Poungpair O, Pootong A, Maneewatch S, Srimanote P, Tongtawe P, Songserm T, Tapchaisri P, and Chaicumpa. A human single chain tranbody specific to matrix protein (M1) interferes with replication of influenza A virus. Bioconjugate Chem 2010; 21:1134-1141. 2. Maneewatch S, Thanongsaksrikul J, Songserm T, Thueng-In K, Kulkeaw K, Thathaisong U, Srimanote P, Tongtawe P, Tapchaisri P, and Chaicumpa W. Human single-chain antibodies that neutralize homologous strains and clades of influenza A virus subtype H5N1. Antivir Ther 2009; 14:221-230. Keywords: Cell penetrating antibody, qPCR, phage display library, Matrix protein-1, mouse model Conference: 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013. Presentation Type: Abstract Topic: Translational immunology and immune intervention Citation: Dong-din-on F, Srimanote P, Pissawong T, Monkong A, Lertwatcharasarakul P, Songserm T and Chaicumpa W (2013). Cell penetrable human ScFv specific to influenza A virus matrix protein, M1, mitigates influenza severity. Front. Immunol. Conference Abstract: 15th International Congress of Immunology (ICI). doi: 10.3389/conf.fimmu.2013.02.01118 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 11 Jul 2013; Published Online: 22 Aug 2013. * Correspondence: Prof. Wanpen Chaicumpa, Mahidol University, Department of Parasitology, Faculty of Medicine Siriraj Hospital, Bangkok Noi, Bangkok, 10700, Thailand, wanpen.cha@mahidol.ac.th Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Fonthip Dong-din-on Potjanee Srimanote Tippawan Pissawong Angkasiya Monkong Preeda Lertwatcharasarakul Thaweesak Songserm Wanpen Chaicumpa Google Fonthip Dong-din-on Potjanee Srimanote Tippawan Pissawong Angkasiya Monkong Preeda Lertwatcharasarakul Thaweesak Songserm Wanpen Chaicumpa Google Scholar Fonthip Dong-din-on Potjanee Srimanote Tippawan Pissawong Angkasiya Monkong Preeda Lertwatcharasarakul Thaweesak Songserm Wanpen Chaicumpa PubMed Fonthip Dong-din-on Potjanee Srimanote Tippawan Pissawong Angkasiya Monkong Preeda Lertwatcharasarakul Thaweesak Songserm Wanpen Chaicumpa Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.