Mucilaginibacter Herbaticus Sp Nov., Isolated From The Rhizosphere Of The Medicinal Plant Angelica Sinensis

A strictly aerobic, Gram-staining-negative, non-motile and rod-shaped bacterial strain, DR-9(T), was isolated from rhizosphere soil of the medicinal herb Angelica sinensis. Strain DR-9T grew at 20-40 degrees C, at pH 4.0-9.0 and in the presence of 0-1% (w/v) NaCl. The major fatty acids were iso-C-15:0 and summed feature 3 (C-16:1 omega 7c and/or C-16:1 omega 6c and/or iso-C-15:0 2-OH), MK-7 was the major isoprenoid quinone, and phosphatidylethanolamine and an unidentified aminophospholipid were the major polar lipids. A phylogenetic tree based on 16S rRNA gene sequences showed that strain DR-9(T) formed a lineage within the genus Mucilaginibacter and was closely related to Mucilaginibacter polysacchareus DRP28(T) (96.1% sequence similarity), Mucilaginibacter myungsuensis HMD1056(T) (95.9% sequence similarity), Mucilaginibacter ximonensis XM-003(T) (95.8%) and Mucilaginibacter boryungensis BDR-9(T) (95.1 %). The status of strain DR-9(T) as a representative of a separate species was confirmed by DNA hybridization, with 38.6, 36.3 and 29.9% DNA-DNA relatedness with M. polysacchareus DRP28(T), M. ximonensis XM-003(T) and M. boryungensis BDR-9(T), respectively. The genomic DNA G + C content of strain DR-9(T) was 49.8%. These data suggest that strain DR-9(T) should be considered as a representative of a novel species of the genus Mucilaginibacter, for which the name Mucilaginibacter herbaticus sp. nov. is proposed. The type strain is DR-9(T) (=KACC 16469(T)= NBRC 108839(T)).