Isolation and characterization of Bacillus subtilis spores that are superdormant for germination with dodecylamine or Ca2+ -dipicolinic acid.
JOURNAL OF APPLIED MICROBIOLOGY(2013)
Abstract
Aims To isolate and characterize spores superdormant (SD) for germination with either Ca2+-dipicolinic acid (CaDPA) or dodecylamine. Methods and Results Bacillus subtilis spores were germinated three times with either CaDPA or dodecylamine and germinated spores removed after each germination treatment, yielding 0 center dot 9% (CaDPA-SD spores) or 0 center dot 4% (dodecylamine-SD spores) of initial dormant spores. Compared to dormant spores, CaDPA-SD spores germinated poorly with CaDPA and better with dodecylamine and nutrient germinants, although release of DPA from individual CaDPA-SD spores was slow during nutrient germination, and this germination was strongly inhibited by TbCl3. The CaDPA-SD spores were sensitive to hypochlorite and had elevated levels of nutrient germinant receptors (GRs) relative to levels in dormant spores. Dodecylamine-SD spores' germination with dodecylamine and nutrients was similar to that of dormant spores, their germination with Ca-DPA was slower than that of dormant spores, and these SD spores' GR levels were lower than in dormant spores. However, dodecylamine-SD spores were not sensitive to hypochlorite, and the nutrient germination of these SD spores was only partially inhibited by TbCl3. Conclusions CaDPA-SD spores appear to have a coat defect and accompanying low levels of the cortex-lytic enzyme CwlJ. The defect in dodecylamine-SD spores, however, is not clear. Significance and Impact of the Study The results suggest that triggering germination by non-GR-dependent germinants is a potential strategy for efficient spore inactivation.
MoreTranslated text
Key words
Bacillus,bacterial spores,Ca-DPA,dodecylamine,spore germination,spores,superdormant
AI Read Science
Must-Reading Tree
Example
![](https://originalfileserver.aminer.cn/sys/aminer/pubs/mrt_preview.jpeg)
Generate MRT to find the research sequence of this paper
Chat Paper
Summary is being generated by the instructions you defined