Complete (1)H and (13)C signal assignment of prenol-10 with 3D NMR spectroscopy.

The complete assignment of H-1 and C-13 chemical shifts of natural abundance prenol-10 is reported for the first time. It was achieved using 3D NMR experiments, which were based on random sampling of the evolution time space followed by multidimensional Fourier transform. This approach makes it possible to acquire 3D NMR spectra in a reasonable time and preserves high resolution in indirectly detected dimensions. It is shown that the interpretation of 3D COSY-HMBC and 3D TOCSY-HSQC spectra is crucial in the structural analysis of prenol-10. Copyright (C) 2009 John Wiley & Sons, Ltd.