[Screening for inhibitor of vascular endothelial growth factor from random peptide library].
Zhonghua zhong liu za zhi [Chinese journal of oncology](2002)
摘要
OBJECTIVE:To screen for the inhibitor of vascular endothelial growth factor (VEGF) 165 from random peptide library.
METHODS:Positive phage clones were rescued after two rounds of panning and competitive elution. Its affinity activity to KDR was monitored through ELISA, immunohistochemical method, Chicken CAM assay and MTT.
RESULTS:Five specific binding positive target molecule phage clones were obtained which were able to bind to cells whose surface had high KDR, among which, clone 3 and 13 could effectively block the vascularization of the chorioallantoic membrane of chick embryo, but they were not inhibitive on the proliferation of high KDR expression cells.
CONCLUSION:The peptides, being the inhibitors of VEGF, may be useful in the treatment of cancers.
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关键词
vascular endothelial growth factor,random peptide library,inhibitor
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