脐血CD34+细胞体外诱导扩增红系细胞的探索

Yan-Jun JIA,Jiang LIU,Ke-Ying ZHANG,Xiao-Yan SHAN,Wei LI,Xiao-Mei HE,Li-Jun WANG,Na LIU,Lin WANG,Shuang CUI,Lei NI,Bo-Tao ZHAO,Zhi-Yin GONG,Dong-Mei WANG,Song-Ming GAO,Zhi-Xin ZHANG

Zhongguo shi yan xue ye xue za zhi / Zhongguo bing li sheng li xue hui = Journal of experimental hematology / Chinese Association of Pathophysiology（2009）

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Abstract

本研究利用脐血CD34+细胞在体外大量扩增红系细胞为解决血源短缺、杜绝输血传染、克服稀有血型患者疑难配血等问题提供一种有益的途径.利用添加SCF、IL-3、EPO等细胞因子的无血清培养液扩增脐血来源的CD34+细胞,并通过间充质干细胞的支持培养来诱导其向红系细胞扩增和分化.结果表明,经过23天培养以后,本方法可以使细胞的扩增倍数达到2.52×105,其中95%以上都是红系细胞,粒单系细胞和巨核细胞所占比例都在1%以内.无间充质干细胞的培养体系在细胞扩增数量及红系细胞所占比例方面都明显逊于有间充质干细胞支持的培养体系.结论:利用细胞因子和间充质干细胞可以在体外大量扩增红系细胞,其中间充质干细胞对红系细胞增殖和分化起重要的促进作用.

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Key words

mesenchymal stem cell,amplification in vitro,erythroid cell,hematopoietic stem cell,CD34+ cell

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