Correlation of cefoxitin MICs with the presence of mecA in Staphylococcus spp.

JOURNAL OF CLINICAL MICROBIOLOGY(2009)

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摘要
This report describes the results of an 11-laboratory study to determine if a cefoxitin broth microdilution MIC test could predict the presence of mecA in staphylococci. Using breakpoints of <= 4 mu g/ml for mecA-negative and >= 6 or 8 mu g/ml for mecA-positive isolates, sensitivity and specificity based on mecA or presumed mecA for Staphylococcus aureus at 18 h of incubation were 99.7 to 100% in three cation-adjusted Mueller-Hinton broths tested. For coagulase-negative strains at 24 h of incubation, breakpoints of <= 2 mu g/ml for mecA-negative and >= 4 mu g/ml for mecA-positive isolates gave sensitivity and specificity of 94 to 99% and 69 to 80%, respectively.
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关键词
methodology,predictive value of tests,genes,in vitro
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