Late sodium current inhibition alone with ranolazine is sufficient to reduce ischemia- and cardiac glycoside-induced calcium overload and contractile dysfunction mediated by reverse-mode sodium/calcium exchange.
JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS(2012)
Abstract
Excessive reverse-mode (RM) sodium/calcium exchanger 1.1 (NCX1.1) activity, resulting from intracellular sodium accumulation caused by reduced Na+/K+-ATPase activity, increased Na-H exchanger 1 activity. The induction of the voltage-gated sodium channel late current component (late I-Na), is a major pathway for intracellular calcium (Ca-i(2+)) loading in cardiac ischemia-reperfusion (IR) injury and cardiac glycoside toxicity. Inhibition of late I-Na with the antianginal agent ranolazine is protective in models of IR injury and cardiac glycoside toxicity. However, whether inhibition of late I-Na alone is sufficient to provide maximal protection or additional inhibition of RM NCX1.1 provides further benefit remains to be determined conclusively. Therefore, the effects of ranolazine were compared with the I-Na inhibitor lidocaine in models of IR injury and ouabain toxicity, RM NCX1.1-mediated Ca2+ overload, and patch-clamp assays of RM NCX1.1 currents. Ranolazine and lidocaine (10 mu M) similarly reduced Ca-i(2+) overload and improved left ventricle work recovery in whole-heart models of IR injury or exposure to ouabain (80 mu M). Ranolazine (10 mu M), but not lidocaine (10 mu M), reduced RM NCX1.1-mediated Ca-i(2+) overload in ventricular myocytes. Furthermore, ranolazine inhibited RM NCX1.1 currents (IC50 1.7 mu M), without affecting forward mode currents, revealing that ranolazine has novel RM NCX1.1 inhibitory actions. However, because lidocaine provides similar protection to ranolazine in whole-heart models but does not inhibit RM NCX1.1, we conclude that induction of late I-Na is upstream of RM NCX1.1 activity and selective inhibition of late I-Na alone is sufficient to reduce Ca-i(2+) overload and contractile dysfunction in IR injury and cardiac glycoside toxicity.
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Key words
Calcium Signaling
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